Journal: Scientific Reports

Article Title: Understanding of the different roles of Noggin in the Noggin-BMP-2 and Noggin-BMP-9 dimer complexes at the molecular level

doi: 10.1038/s41598-025-33735-8

Figure Lengend Snippet: Initial and final structures of Noggin-BMP-2 and Noggin-BMP-9 after 100 ns MD simulation. Identification of the binding effect of BMP-2 and BMP-9 to BMPR2 in MC3T3-E1 Cells by Noggin using Immunoprecipitation. ( A ) Initial structures of Noggin-BMP-2 and ( B ) Initial structures of Noggin-BMP-9 ( C ) Aligned structures of initial (White color) and final structures of Noggin-BMP-2 (Coral/Blue) ( D )Aligned structures of initial (White) and final structures of Noggin-BMP-9 (Green/Ultraviolet) ( E ) RMSD of Noggin-BMP-2 and Noggin-BMP-9 complexes during the all-atom MD simulation (100 ns). ( F ) RMSF (Root Mean Squared Fluctuation) of Noggin-BMP-2 and Noggin-BMP-9 complexes during the all-atom MD simulation (100 ns). RMSD and RMSF values ​​represent the mean and standard deviation of three independent simulations. ( G ) Western blot analysis using total cell lysates. ( H ) Immunoprecipitation (IP) of BMPR2 followed by immunoblotting (IB) to detect BMP-2 and BMP-9 binding.

Article Snippet: The mouse pre-osteoblastic cell line MC3T3-E1 was purchased from the American Type Culture Collection (ATCC, CRL-2593, Manassas, VA, USA).

Techniques: Binding Assay, Immunoprecipitation, Standard Deviation, Western Blot

Journal: Scientific Reports

Article Title: Understanding of the different roles of Noggin in the Noggin-BMP-2 and Noggin-BMP-9 dimer complexes at the molecular level

doi: 10.1038/s41598-025-33735-8

Figure Lengend Snippet: Identification of binding effect of BMP-2 and BMP-9 to BMPR2 in MC3T3-E1 cells by Noggin. ( A ) BMP-2–treated group, ( B ) BMP-2 + Noggin–treated group, ( C ) BMP-9–treated group, ( D ) BMP-9 + Noggin–treated group. DAPI (blue), anti-BMPR2 (red), and anti-BMP-2 or anti-BMP-9 (green). Merged images show co-localization (yellow). Scale bar: 10 μm; magnification: ×600. ( E ) Western blot of SMAD1/5/9 phosphorylation under the indicated treatments. Top, p-SMAD1/5/9; middle, total SMAD1/5/9; bottom, GAPDH. ( F) Alkaline phosphatase (ALP) activity measured 60 min after BMP addition using a p-nitrophenyl phosphate colorimetric assay (405 nm). Bars indicate mean ± SD ( n ≥ 3). p < 0.0001 vs. NT for all treated groups; asterisks denote significance relative to NT.

Article Snippet: The mouse pre-osteoblastic cell line MC3T3-E1 was purchased from the American Type Culture Collection (ATCC, CRL-2593, Manassas, VA, USA).

Techniques: Binding Assay, Western Blot, Phospho-proteomics, Activity Assay, Colorimetric Assay

Journal: Gels

Article Title: Whey Protein Isolate Hydrogels Containing Cannabidiol Support the Proliferation of Pre-Osteoblasts

doi: 10.3390/gels11060418

Figure Lengend Snippet: The results of % cell viability and proliferation of MC3T3-E1 pre-osteoblasts seeded on WPI/CBD hydrogels relative to WPI0 (set as 100%). TCPS is tissue-culture-treated polystyrene and serves as control. Absorbance readings at 570/600 nm were taken at days 3 and 7. Each bar represents the mean ± SD of n = 6 (** p < 0.01, **** p < 0.0001; compared to the WPI control) ( ## p < 0.01, #### p < 0.0001; compared to the TCPS control).

Article Snippet: Cellular viability and behaviour assays were conducted utilising the mouse calvaria osteoblast precursor cell line (MC3T3-E1, DSMZ Braunschweig, Germany, ACC-210).

Techniques: Control

Journal: Gels

Article Title: Whey Protein Isolate Hydrogels Containing Cannabidiol Support the Proliferation of Pre-Osteoblasts

doi: 10.3390/gels11060418

Figure Lengend Snippet: SEM images demonstrating the cellular morphology of MC3T3-E1 pre-osteoblasts on WPI/CBD hydrogels. Images were taken at 7 and 10 days of culture ( middle and lower panels ). The upper panel shows unseeded hydrogels (without cells) to facilitate comparison. Scale bars represent 10 μm.

Article Snippet: Cellular viability and behaviour assays were conducted utilising the mouse calvaria osteoblast precursor cell line (MC3T3-E1, DSMZ Braunschweig, Germany, ACC-210).

Techniques: Comparison

Journal: Gels

Article Title: Whey Protein Isolate Hydrogels Containing Cannabidiol Support the Proliferation of Pre-Osteoblasts

doi: 10.3390/gels11060418

Figure Lengend Snippet: Assessment of the osteogenic potential of MC3T3-E1 pre-osteoblastic cells cultured on WPI/CBD hydrogels; ( a ) the expression of normalised ALP-specific activity on days 3, 7, and 14, ( b ) collagen production, and ( c ) calcium production by pre-osteoblasts on days 7, 14, and 21. Each bar represents the mean ± SD of n = 6 (* p < 0.05, ** p < 0.01, **** p < 0.0001; compared to the WPI0 control) ( # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001; compared to the TCPS control), while the absence of asterisks in the graph of calcium production ( c ) signifies statistically non-significant differences when compared to the WPI0 control.

Article Snippet: Cellular viability and behaviour assays were conducted utilising the mouse calvaria osteoblast precursor cell line (MC3T3-E1, DSMZ Braunschweig, Germany, ACC-210).

Techniques: Cell Culture, Expressing, Activity Assay, Control